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(A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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(A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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(A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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(A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

Journal: bioRxiv

Article Title: Most early-born subplate neurons persist as Layer 6b neurons in the adult mouse neocortex

doi: 10.1101/2025.11.20.689634

Figure Lengend Snippet: (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

Article Snippet: Cleared whole brain tissues were imaged by Light sheet microscopy (Olympus, MVX10-LS), and the z-axis interval was 4 μm in the P0 sample and 9 μm in the 8 weeks.

Techniques: Immunohistochemical staining, Expressing, Fluorescence, Microscopy